Calibration of 6460 triple quad instrument

Given that your 322 peak is showing up as 311 with the other ions only marginally outside their calibration window, I would suggest that that you have a contaminant in the tune mix (calibrant solution). I would dispose of the current solution, wash out the bottle with LCMS grade ACN and then replace with new tune mix (P/N G1969-85000). Then run an Autotune in both polarities. Come back on this thread if the problem continues or changes form. Cheers, Danny

Given that your 322 peak is showing up as 311 with the other ions only marginally outside their calibration window, I would suggest that that you have a contaminant in the tune mix (calibrant solution). I would dispose of the current solution, wash out the bottle with LCMS grade ACN and then replace with new tune mix (P/N G1969-85000). Then run an Autotune in both polarities. Come back on this thread if the problem continues or changes form. Cheers, Danny

Thank you Dr. Danny for your detail suggestion. Sincerely, Yogesh 

Dear Dr. Danny, 

After doing the above suggestion, these messages are came 

Message-1 came first and then after few seconds 2nd one. 

After closing these message calibration get aborted. 

Please let us know if MS need servicing?

Hi 

Pls do autotune by default factory tune.

If still problem persist then better to call your local agilent support..