Infinity 1260 II vialsampler standards dilution calibration programm

Hi,

there is no a downloadable program at the moment. But you could take a look at the Application Note Let Your Autosampler Do Your Pipetting (agilent.com). It describes the sampler programming approach to create the dilution series for calibration purposes.

Hi thanks for your response, but  there s no sequence example in this application note and description of the different functions in autosampler manual are very simple.
Is there any other manual?

regards.

L Stephan

Hello, are you looking for an injection sequence or something different? Figure 2 shows an example, it is in OpenLab, but it is not much different to ChemStation in this sense. In general, are you looking for injector programming information or how to do calibration analysis in the software, or both?

Hello,
I m trying to work with the application note "Let your autosampler do your pipetting". So i see how it functions for the first calibration vial but i ask me how it functions for the next calibration vials?

The injector program is applicable just for the first calibration vial or for all?

In the sequence, i see the injector program associated to the second line but not to the lines 5-10 calibration levels.

Is you could help me?

Thanks

Sure, I can help. You are right, Table 1 shows only a fraction of the whole injector program. But at the same time it is a block/group of commands which you need to repeat for every dilution step. In the Application Note they have 7 dilutions, they would need to repeat this block 6 more times. All saved as one sample preparation method. Sample preparation method is shown only once in Figure 2. Therefore, all sample dilutions are done in advance, prior the first sample analysis. For every repetition of that block, you need to change 2 things: the vial where you eject and mix the stock and the diluent, so, instead of ejecting into D1F-D2, the next block would eject into D1F-D3, the next one - to D1F-D4 and so on. The second thing to change is the position to draw the stock. The first draw (Table 1) is done from the diluent (D1F-E1) and the 100 µg/mL stock (D1F-D1), the second draw would be from the diluent (D1F-E1) and the already prepared "1:5" dilution from D1F-D2. The next one, will be again the diluent (D1F-E1) and the previous sample, D1F-D3. This way, every next sample is 5-fold more dilute than the previous. The volumes drawn would remain the same at every repetition of the block.
On a side note, the seventh dilution was treated as a sample and not used for calibration, as well as the original stock.

Sure, I can help. You are right, Table 1 shows only a fraction of the whole injector program. But at the same time it is a block/group of commands which you need to repeat for every dilution step. In the Application Note they have 7 dilutions, they would need to repeat this block 6 more times. All saved as one sample preparation method. Sample preparation method is shown only once in Figure 2. Therefore, all sample dilutions are done in advance, prior the first sample analysis. For every repetition of that block, you need to change 2 things: the vial where you eject and mix the stock and the diluent, so, instead of ejecting into D1F-D2, the next block would eject into D1F-D3, the next one - to D1F-D4 and so on. The second thing to change is the position to draw the stock. The first draw (Table 1) is done from the diluent (D1F-E1) and the 100 µg/mL stock (D1F-D1), the second draw would be from the diluent (D1F-E1) and the already prepared "1:5" dilution from D1F-D2. The next one, will be again the diluent (D1F-E1) and the previous sample, D1F-D3. This way, every next sample is 5-fold more dilute than the previous. The volumes drawn would remain the same at every repetition of the block.
On a side note, the seventh dilution was treated as a sample and not used for calibration, as well as the original stock.

Thanks a lot,
I will try to apply this.

One thing would be interesting 
Perharps, a command like to this block for all positions vials +1, so this program will repeat for an fix number of vials define in method :-)
Just a suggestion :-)

Regards.

L Stephan

Sorry nut on sequence table, i dont have any column sample prep method. 
I tried to add this column from fields available but it s no appear in the list of fields.

Any idea?

Thanks

Injector program (sample prep method in OpenLab) is a part of the analytical method in ChemStation. You would need to create a second method, "biased" to the sample preparation. This method would need to include the first injection at the end of the repeated blocks (from my previous post). To add the injector program, you need to go to "Edit Entire Method" select "Instrument/Acquisition" and on the Multisampler Injector Program tick the "Use Injector Program" and configure the repeated blocks for dilution prep and the first injection (specify the vial). In the sequence, you have to use 2 methods. They have to use the same analytical part (injection volume, flow rate, gradient, temperature, wavelength, etc.), but the very first has to Use Injector Program activated and configured, in the second one injector program has to be deactivated. Also to have the injector program working, you have to specify an injection vial in the Injection Table for the first method. But this vial will be ignored, the sample won't be drawn from there. It is only needed to make the line considered as a real sample and not a blank.

Good luck! :)

Thanks for all those explications, it seems to be complex but i will try to succeed 

Many thanks for all that :-)
In fact we re limited by the volume of the loop 100µ so we need to add glass inserts into vials.

But is there another way, like draw x µL of stock standard solution in loop eject it in a vial and add mobile phase volume based on default methode mobile phase flow (depend on time) in order to dilute it as necessary in vial?

Or we must using glass inserts?

Regards 

L Stephan

If I understood correctly, you would like to use the mobile phase coming from the pump to perform the dilution. Unfortunately, it is not possible. The system is designed to protect the column from sudden pressure changes. Therefore, it cannot be in the main pass and be connected to the atmosphere (pre-column). All programmed valve switches (in injector program) are done with the needle in the needle seat.

So, glass inserts are the way to go in this case.

It depends on your workflow and how much you need to repeat these dilutions. There are automation solution like an Automated Liquid Handling from Agilent, Automated liquid handling, compact and flexible liquid handler | Agilent.

Cheers!