Column for metabolites

Hi Chiara 

Maybe one of the HiPlex range of columns might work for you?

https://www.agilent.com/cs/library/applications/5990-8801EN%20Hi-Plex%20Compendium.pdf

Kindest regards

Andy

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Hi Andy, 

Yes, thank you should be fine that one :) What you think about also InfinityLab Poroshell 120 HILIC, 4.6 x 250 mm, 4 µm ( 690970-901 | Agilent ) ?

Kind Regards,

Chiara

Hi Chiara 

My concern would be that the HILIC column might not retain ethanol.  For the other analytes, HILIC might be a possibility.  What are you using for detection with this analysis?

Regards

Andy

Hi Andy, 

I am now using Aminex HPLC_87H Column 300x 7.8, but I would like to see if by using other columns I can improve the method we currently have in use. In particular I would be interested in the detection of phosphate. I am considering trying different columns:

• NUCLEOGEL SUGAR 810 H, 8-10 µm, 300x7.8 mm
• InfinityLab Poroshell 120 HILIC, 4.6 x 250 mm, 4 µm,
• Organic Acid Length x ID : 250 x 8 mm
• and possibly the one you recommended Agilent Hi-Plex H, 7.7 × 300 mm, 8 μm

.. of these I want to understand which one(s) to order and test..

Best, 

Chiara

Hi Andy, 

I am now using Aminex HPLC_87H Column 300x 7.8, but I would like to see if by using other columns I can improve the method we currently have in use. In particular I would be interested in the detection of phosphate. I am considering trying different columns:

• NUCLEOGEL SUGAR 810 H, 8-10 µm, 300x7.8 mm
• InfinityLab Poroshell 120 HILIC, 4.6 x 250 mm, 4 µm,
• Organic Acid Length x ID : 250 x 8 mm
• and possibly the one you recommended Agilent Hi-Plex H, 7.7 × 300 mm, 8 μm

.. of these I want to understand which one(s) to order and test..

Best, 

Chiara

HI Chiara

Of all of those columns, the HILIC is the most different to the others and might offer quite different selectivity.  Whether it would retain all of the analytes you mention, I cannot say.

Kindest regards

Andy

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