Anyone can help with my question

 How to Create an Internal Standard Calibration Curve in OpenLab ChemStation 

when you watch the video above, you will see the graphs in the below picture

my question is what causes the zigzag pattern, the pressure? 

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  • thank you for the reply.

    when we  run samples, we sometimes got really weird things. can you help me further what the problem could be. the picture below is just a water sample

    also the following picture looks similar to the pattern shown in my first question? 

    this one is a brine water sample

    is it because we run brine water on HPLC and it has caused the system too dirty? 

    thanks so much

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