I am having trouble in stabilizing my RID detector. Please provide me proper procedure for stabilization and getting good baseline in RID. Also when i am joining the column or removing the column there is no effect on the RID baseline.
Please follow Below Procedure for RID Optimization.
Follow these thirteen points to optimize the performance of your refractive index detector.
1 Position the solvent and waste reservoirs correctly
And as a reminder:
- never ever operate an RID without a proper degasser. All Agilent degassers can be used with the exception of G1379A and G1379B.
- always have a TCC/MCT in your stack
- always have to…
All the important steps can also be found in the manual. No reaction on the baseline COULD point to a faulty purge valve. Keep pumping with fresh, clean water and switch the purge valve repeatedly. Maybe…
Yeah notice I didn't say "..and keep that bottle exposed to light and let the water sit for weeks on end."
Consider RI for water v. temperature and the y axis units of the RID. My opinion was developed over years of working on RIDs, including many successful RID OQs with less than ideal environmental conditions.