G1364E Delay in Fraction Collection

Hi everyone, 

I have some problems with a 1260 Infinity II Prep HPLC system. 
I updated the firmware earlier this week because we had troubles with the needle piercing the bottom of the safety wash port, resulting in holes and leakage from the needle wash. 
I was told that it was a known firmware issue, and therefore updated the system. 

Now we see a delay in our fraction collection (see attached photo) when using peak-based collection. 
I thought the delay was a good thing corresponding to the delay between the detector and fraction collector, but we have not previously experienced this delay. 
When we set the delay value to "off" instead of "as calibrated" we still see this delay. The system has not been calibrated while I have been working with it, and we have normally collected the fractions without any delay. 

Does anyone have any suggestions of how to solve our problem? We have a G1364E fraction collector.

Thank you in advance!

Parents
  • Hey, has the method that you have been using been changed? It looks like you are using peak detection of "slope", but have something high for the up slope in the analytical method; therefore, during the upward part of the peak you are not collecting the fraction. As you are going downwards, it seems to be okay indicating that the down slope parameters are okay. Here is an example.

Reply
  • Hey, has the method that you have been using been changed? It looks like you are using peak detection of "slope", but have something high for the up slope in the analytical method; therefore, during the upward part of the peak you are not collecting the fraction. As you are going downwards, it seems to be okay indicating that the down slope parameters are okay. Here is an example.

Children
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