Infinity 1290 autosampler (G4226A). Initialization failed.

Hey, See: Agilent 1290 Infinity Autosampler User Manual on page 108. There is a breakdown of the possible causes and solutions. From my experience, this is often caused by the needle not being properly aligned inside of the carrier. When I was learning how to do the maintenance needle replacement, I sometimes caused this error by having the needle not centered in the carrier. The sampler could also require an alignment procedure as stated in step 1. 

Hey, See: Agilent 1290 Infinity Autosampler User Manual on page 108. There is a breakdown of the possible causes and solutions. From my experience, this is often caused by the needle not being properly aligned inside of the carrier. When I was learning how to do the maintenance needle replacement, I sometimes caused this error by having the needle not centered in the carrier. The sampler could also require an alignment procedure as stated in step 1.