UHPLC, need of use of eluent 0.1micron filtered

Hi

there is no need for higher grade  , 0.2 is enough, 

also porous less than 0.2 may adsorb significant percentage of your analyte of your apply it on your analyte.

in case of mobile phase, absolutely no need for it.

even LC MS doesn't need more than 0.2, but i bet you are facing buffer perciptation issue, 

it is usuallydue to compositionof mobile face, it is post mixer issue when compositionof Mphs percipitate in Lines after mixing

Hi

there is no need for higher grade  , 0.2 is enough, 

also porous less than 0.2 may adsorb significant percentage of your analyte of your apply it on your analyte.

in case of mobile phase, absolutely no need for it.

even LC MS doesn't need more than 0.2, but i bet you are facing buffer perciptation issue, 

it is usuallydue to compositionof mobile face, it is post mixer issue when compositionof Mphs percipitate in Lines after mixing

hi Mohammad, thank you for your hint. Actually precipitation of buffers is not an issue but I'll keep it in mind: why you're suspicious about it? is there a relation to dissolved particles smaller than 0.2 microns and buffer precipitation?

kind regards,

Emanuele

Hi Emanuele

most suspended particles are more than 0.2 um 

only some sub-bacterial are sub 0.2 um which isn't a big concern

there is no direct relationship between  dissolved particles smaller than 0.2 microns and buffer precipitation

...

Buffer precipitation occurs when mixing two  completely dissolved (organic solvent )and (buffer in water)

it differs due to ratio ,pH , buffer type and buffer concentration

Filtration doesn't make any significant difference

Hi Mohammad, very clear and fruitful answer: thank you. Wish you a nice night, Emanuele