Problem with "mix" command on injection program

Hi avanoc

Since the metering device only has a volume of 100µL, and you have drawn 2 x 50µL, there's no volume in the metering device to allow you to mix.  What options for mixing location do you have on the 1260?  I'm not sure where the 1100 autosampler would do the mixing.

/Andy

Thank you Andy

Both 1260 and 1100 injection programs shows the same options:

*Draw: (default / maximum / x μL) from (sample / location x / seat / air) speed (default / maximum / x μL) & offset (default / x mm).

*Mix: (default / maximum / x μL) from (seat / air) speed (default / maximum / x μL) & repeat(x times).

I tried every combination possible in both softwares, with the same result: 1100 allows me to mix a combination of volumes from different vials up to 100 μL, but 1260 does not. 

§ Sol §

Hi avanoc

Does the 1100 have an additional loop between the needle seat and the injection valve?  These are typically used in multi-draw operation to allow injection volumes greater than 100uL.

/Andy

Hi avanoc

Does the 1100 have an additional loop between the needle seat and the injection valve?  These are typically used in multi-draw operation to allow injection volumes greater than 100uL.

/Andy

Please check the sample loop size and metering pump size as mentioned above in the different systems,  Can you share the injection program that you are using?  Sometimes an Eject Max as the first line helps by zeroing the metering device..

AndyG

Hi Andy

There's no additional loop that I know of. Agilent's representative tech service in Argentina (Analytical Technilogies), claims that the 1100's injector is different from the 1260's model. He said that this configuration difference is the responsible for this issue.