RID stabilizes and destabilizes around 10 mins after

Hi,

I am having some problems with the stabilisation of my RID.

I have already applied the suggestions mentioned in previous questions:

https://community.agilent.com/technical/lc/f/forum/711/rid-stabilization

I had to optical balance, but now the problems is that detector stays stabilized during ~10mins (green - Ready) and then turns yellow again. Opening the purge for around 2-3 mins makes the instrument ready again althought it doesn't last more than 10 mins.

Anyone can tell me what could be the problem please?,

Thank you

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ISH over 2 years ago +1 verified

Hi You can flush the system with low flow whole night and then check. I assumed degasser , mobile phase( not contaminted)and temp. control are okay. Optical balance later. And after this, still…

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0 Wolney Vasconcelos over 2 years ago

Hi,

Do you still have this problem?

Your flow cell could still be contaminated and one option to decontaminate is to use a syringe with water and carefully insert it directly into the flow cell when it is both in recycle mode and (the other one). You might need to repeat it 2 or 3 times. Be careful to not contaminate it again when replacing the solvent.

Regards,
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0 Wolney Vasconcelos over 2 years ago

Hi,

Do you still have this problem?

Your flow cell could still be contaminated and one option to decontaminate is to use a syringe with water and carefully insert it directly into the flow cell when it is both in recycle mode and (the other one). You might need to repeat it 2 or 3 times. Be careful to not contaminate it again when replacing the solvent.

Regards,
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