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HPLC ISSUE

Carol_Garcia

Hi,

I have an issue 1100 HPLC. The retention time is shifting. I changed the station pump, the needle, the needle seat, the column, the seal rotor, and the guard. The issue continues. Some ideas and recommendations.  

Thanks

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    Hi Carol,

    When you say RT is shifting, is it longer and constant, shorter and constant or moving back and forth?

    What is your pump model and are you running a gradient?    

    Checking the flow rate as previously mentioned is a good idea but if your pressure is steady under isocratic conditions, then it a good assumption your check valves are okay.  At that point, you can still have a leak so checking the flow will confirm whether to check for a leak or not.

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  • 0

    Hi Carol,

    When you say RT is shifting, is it longer and constant, shorter and constant or moving back and forth?

    What is your pump model and are you running a gradient?    

    Checking the flow rate as previously mentioned is a good idea but if your pressure is steady under isocratic conditions, then it a good assumption your check valves are okay.  At that point, you can still have a leak so checking the flow will confirm whether to check for a leak or not.

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