Load and Lock prep column system

Does anyone have any experience with AGilents Load and Lock prep column system?  Used for loading your own prep columns with bulk support?  Are these easy to use?  Comparable to purchasing prepacked prep columns?



  • Yes. They are easy to use. Columns will last longer than pre-packed and will maintain high performance through it’s entire lifetime.

    Approx. 25% higher loading than pre-packs or competitor’s pack your own.

    Equal to or better performance than pre-packs or competitor’s pack your own.

    Cost savings will be dependent on how often one goes through columns and time savings due to less runs per campaign.

    Here is more info that may be helpful to you as well.

    Purify your way-Agilent Load and Lock Columns

    Agilent Load &Lock Columns-Data Sheet

  • The lab scale L&L Columns and Packing Station are designed to get your customers quickly up and running, to pack their own high performance liquid chromatography columns. It combines excellent packed bed stability with enhanced flow distribution to the deliver the highest quality purification with maximum speed, flexibility, and ease-of-use. Sample loading is up to approx. 20%+, saving time and reduced your operation costs, because more material can be purified per time unit or smaller inner diameter columns can be used due to higher loadibility. 

    The flexibility of having dual mode packing formats ensures the delivery of consistently high performance results by using dynamic or static axial compression (DAC/SAC). The Load & Lock Column Packing Station also offers the ability to pack an unlimited number of 1 in., 2 in. and 3 in. ID columns, because the column plunger can be locked in the compressed mode, and the prepacked column can then be removed from the packing station.  Then the packing station can be used to pack or unpack other columns. For packing one of the  Load & Lock Columns you need at least one packing station (PCG93LL123STAND) it is all depend in which mode you want to the Load & Lock Column; Static Axial Compression (SAC) OR Dynamic Axial Compression (DAC).  


    Axial compression is used in the column packing process to compress the sorbent particles into a tightly packed bed. There are basically two different forms of axial compression used in column technology today – Static Axial Compression (SAC) where the column is compressed and the plunger is locked in a static “holding” position with a locking mechanism, and Dynamic Axial Compression (DAC) where the packed bed is constantly compressed while being used.

    • Operating in the SAC mode – one packing station will be sufficient. Most customers using the SAC mode.
    • Operating in the DAC mode – each column requires a packing station.



    • A proprietary high performance fluid distribution system that provides an enhanced performance of the column, diffusing the sample more efficiently over the complete bed surface. This design has been proven to minimize back pressure and peak band broadening, while enhancing sample loading (column productivity) and the useful life of the frits and distribution plates.
    • A quick release single bolt clamp on the outlet end of the column for fast and easy packing and unpacking.
    • Provides both Static Axial Compression (SAC) + Dynamic Axial Compression (DAC).
    • The SAC system allows for the “gentle” hydraulic compression of sorbents, preventing the crushing of sensitive particles (esp. 300A particles or polymers) due to excess packing pressures seen with the typical DAC mode of packing columns. After the gentle compression in accomplished, in the SAC mode, the plunger can be “locked” into a “holding” position to maintain the compression on the packed bed, or the column can remain on the packing station with the air pressure applied to facilitate the DAC mode of operation.
    • The SAC mode safe-guards the user from possible over pressuring from the HPLC system fluid pressure, which can cause the column to literally unpack itself; a common occurrence in typical DAC columns.   
    • Once the packed column plunger is “locked”, the column can be “undocked” from the packing station, thus allowing multiple columns to be serviced by a single packing station.
    •  The “undocking” feature facilitates the use of the packed columns anywhere in the facility without need to transporting the packing station along with the column to the separation site.  
    •  The ability to easily pack and use (SAC/DAC) lab columns of variable inner diameters (2.7 / 5.0 / 7.5 cm) and bed lengths, with the widest possible array of sorbent types (not linked to a specific sorbent - all commercial available sorbents can be packed into our L&L Columns), all on one packing station.
    • The provision for water jackets on the columns provides for column temperature control (more details below).



    Water Jacket:

    An HPLC column Water Jacket is the most effective and lowest cost technique for column temperature control.  When the enclosure is connected to a hot or cold water source, such as a circulating bath, it provides accurate and inexpensive temperature control of the preparative LC column. This is most useful when the separation is temperature dependent or when processing thermally sensitive samples.


    The Stainless Steel Water Jacket is directly welded to the L&L column and cannot be removed from the column body. Connection to the circulating bath is easily made with either 1/4 in. rubber or metal tubing. Connecting tubing is not supplied. The body of the water jacket is made from 316L SS. The entire column, including end fittings, is contained within the Water Jacket. In normal use the liquid is circulated from the bottom of the column toward the top. The top exit port should not be restricted and should flow freely to a drain or recirculation tank. Maximum allowable temperature is 60 °C.

  • 1. Can you please help me with the PN for the first-time order of 500 × 27 mm column?
    2. Without MultiPacker Station is it possible to pack the column?
    3. which Items are included in a spare parts kit?

  • Hello,
    See my comments below in highlighted blue.
    Best regards,
  • Hello,
    See my comments below in highlighted blue.
    Best regards,
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