Good afternoon everyone,
I have a doubt about AutoTune and Tune Evaluation.
I had read somewhere that calibration curves had to be redone as soon as an AutoTune was performed. Therefore I believed that if I would only check the tune with a Tune Evaluation, I would not lose the detector's parameters as long as everything was OK in the report. If not, I would do an AutoTune.
Tune Evaluation reports have been OK for the last 6 months but I recently discovered the MSD sensitivity had dropped drastically, by injecting the same GROB mix injected 6 months ago.
So I believe a Tune Evaluation does not check for abundance. After doing an AutoTune, the EM Voltage passed from 1053 to 1241 and sensitivity improved.
All this because I suddenly lost peaks in the middle of a chromatogram for the same sample.
Is there a way to maintain the MSD response constant over time without doing new calibration curves?
Thank you.