GCMS tuning stopped 5975C

Hello All,

I'm seeing an issue that I haven't come across before - have just changed the column and pumped back down yesterday. Came to do an air and water check this morning and i get the below: 

Tune action stopped:-222{SP00}:MSC:RDY ON Parameter ento value 64.256 out of range [0.000, 64.000]

I didn't do anything with the source yesterday, just changed the column....

Thanks in advance!

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  • Hi ISH, yes all was fine with column installation and pump down was efficient. The issue seems to be with the entrance lense offset - I loaded a previous tune and managed to run an autotune however it is failing due to too high 503/502 ratio. I've seen some advice that this could be due to HED needing cleaning or a dirty source perhaps? The Ion focus was consistently 90.2 however in the latest tune it is now 71.2 so something is not right this week from last week and previous tunes!

    Tune last week had 503/502 ratio of 10.13 where as today it is 13.28. The peak shape of the 502 is also not as neat, slight splitting in the 503 peak and noisier baseline either side.

    If anyone has any advice it would be welcome!

  • Although the tune file and tune evaluation has passed today I have run a methanol blk just to see what the chromatography looks like and it isn't great...see attached. Is this an indication that the source needs cleaning?! This is a relatively new 624 column and hasn't really had many samples down - just some solvents for retention time as I'm trying to set-up a Residual solvents method....so there shouldn't be an issue with the column. 

     

  • Hi louh,

    You have a problem in the analyzer. Response at every mass is occurring. It also shows in your tune report with 800 plus peaks in the background. I would suggest speaking with Agilent Service and setting up a service call. There are a couple of possibilities that could be causing the issues.

    Regards

    James

  • Hi James_jenkins,

    We got the system re-conditioned (second hand) last Aug and so we don't have an Agilent engineer. Would cleaning the source not help (all the online research I've done seems to point to this). Thanks for response.

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