I am new to GC. I am trying to make a calibration table for a set of fatty acid standards using Agilent 8860 (GC-FID)
After the derivitization step, analysis of the resulting methyl esters shows only the solvent peak. Usually a very high solvent peak of about 700000 pA in the blank (heptane). This is also the only peak seen in the analysis of my samples. I mostly start the derivitization with concentration ranges between 5-1000ng/ML. What could potentially be the reason I only observe the solvent peak? Could it be that there is something wrong with the derivitization step or instrument conditions. Thank you.