Volume Accuracy Failure - 850-DS Dissolution Sampling

Hello Suzany,

Please see the Volume Calibration section of the user manual for details:

https://www.agilent.com/cs/library/usermanuals/public/70-9074_EN.pdf

Regards,

Lycrecia

Hi Lycrecia,

We checked the volume calibration section and we did the full range volume dispense, as described in the manual. However, we are not sure which value we should add to the actual volume in the volume calibration screen. Do we need to do the average volume for each range tested (0.1, 0.5, 2, 4, 10 and 15mL)?

How can we do this for only one position (position #2) that failed? 

Thank you!

Here is a summary of the procedure: 

The volume calibration procedure is performed by weighing clean, dry test tubes or 2mL vials and placing them into the specified row of the appropriate sample tray. Select a volume to dispense into the test tubes or vials by entering a value in the mL to dispense field. Press Prime to fill the sample lines and then Dispense to start the dispensing process. When dispensing is complete, remove the sample holders and weigh them again. Using the net volume and density of the media, calculate the actual volume dispensed. Enter this value in the Actual mL field. A corresponding mL/stroke value will be calculated. This is the calibration value that will be used for subsequent pumping. 

Here is a summary of the procedure: 

The volume calibration procedure is performed by weighing clean, dry test tubes or 2mL vials and placing them into the specified row of the appropriate sample tray. Select a volume to dispense into the test tubes or vials by entering a value in the mL to dispense field. Press Prime to fill the sample lines and then Dispense to start the dispensing process. When dispensing is complete, remove the sample holders and weigh them again. Using the net volume and density of the media, calculate the actual volume dispensed. Enter this value in the Actual mL field. A corresponding mL/stroke value will be calculated. This is the calibration value that will be used for subsequent pumping. 

Hi Lycrecia! Thank you somuch! We will try this and I let you know if it works. Thank you!

Hello Suzany,

Were you able to resolve this issue?

Thank you!