I have Run 2 Samples (pkst1 and pkst2) of Total RNA extracted from bacteria. It should have small RNAs along with mRNAs. The samples were quantified with QUbit4.0 and have 28ng/ul. The Total Nano-prokaryote kit was used for quantification. Result showed that there is "unexpected signals in 5S region". No "RIN" value was estimated though rRNA-Ratio was 0.4 and 0.5.
I have Ran the baseline correction and Ladder result was good.
Pls suggest how to resolve the results and get RIN values?