Hi Kenforce,
In this case, I would run the pressure decay test, as you could have a small leak that is causing the inconsistency. I would also consider increasing your oven equilibration time by a 0.5min or so, just to ensure the column phase is at…
Hi Marty,
We have a FID and an MS connected to the same system. We only want to acquire the signal of FID, but if we want to report the method parameters we are “forced” to acquire both (we do not really know why and we have already ask the question…
Sorry for the delayed response. I've found that either works fine. I typically try to keep injection volume low (0.2-1 uL for 1uM protein). I've mostly been using 50mM HEPES 150mM NaCl and get great deconvolution results, even with a 3 min short method…
I am getting message "draw command failed, EE34205" while running batch in G7129B infinity 2 autosampler. I have tried replacing transport assembly, but error is same. I have used another module G7129A Infinity autosampler with this LC, but same error…
How do you set up an auditory sound alert for when a method run completes? I was thinking it may have been a runtime event but I I can’t see my to find it .I set a alarm on my phone for the length of the experiment to remind myself to go check and set…
Lines 3-5 (blank, blank, 1/100ppb) make me think there is a contamination issue. Check the cps of Ni on those first 4 blanks. If you see a downward trend, that could be an indication of a Nickel contamination somewhere in the instrument that is washing…
For Research Use Only. Not for use in diagnostic procedures.
Here is a "best of" collection of links to manuals and tools for Agilent Automated Electrophoresis products (Bioanalyzer, TapeStation, Fragment Analyzer, ZAG DNA Analyzer, Femto Pulse, Oligo…
Hello,
My name is Ben Niemi from the New Hampshire Public Health Laboratories. We are currently running 6470 QQQ LC-MS/MS with a 1260 Infinity II LC Stack.
Recently we've encountered some condensation issues in our column compartment on the line that…
This Information Applies To: 1100, 1200 and 1260 Infinity I and 1260 Infinity II LC Pumps (Excluding preparative pumps with electromagnetic purge valve (EMPV): G1361A. Not applicable to any of the 1290 Infinity II modules with automatic purge valve: G4220X...
This Information Applies To: Agilent 1260 Infinity G1315x DAD or G1365x MWD Modules
Issue:
Removing and re-installing Visible and UV lamps in Agilent 1260 Infinity G1315x DAD or G1365x MWD Modules.
Both the visible and UV lamps are consumable...
This Information Applies To: Agilent 1260 Infinity Binary, Quaternary or Isocratic Pumps.
Issue:
Removal of the pump head from the pump metering drive for maintenance on an Agilent 1260 Infinity Binary, Quaternary or Isocratic Pump Module.
Steps...
This Information Applies To: All Agilent 1260 Infinity Binary, Quaternary, or Isocratic Pumps without the Seal Wash Option installed.
Issue:
Worn pump seals can shed small particles that can cause blockages and pressure issues downstream of the...
This Information Applies To: Agilent G7167x Multisampler modules
Issue:
Required when:
The needle seat is visibly damaged, blocked, or is leaking.
Leaks or blockages in the Vialsampler are observed, especially between the needle and needle...
This Information Applies To: Agilent 1260 Isocratic (G1310B) Quaternary (G1311B) Pumps.
Issue
Required when:
The inlet valve is defective.
The pressure or leak test in Lab Advisor suggests the inlet valve may require replacement.
A large...
This Information Applies To: Agilent 1260 Isocratic (G1310B) quaternary (G1311B) pump modules
Issue:
Required when:
The outlet valve is defective or leaking.
The pressure or leak test in Lab Advisor suggests the outlet valve may require replacement...
This Information Applies To: Agilent 1260 Isocratic (G1310B) quaternary (G1311B) pump modules
Issue:
The seal wear in procedure is important as it acts to "bed-in" the seals to ensure a leak free and pressure-tight pump head.
Required when:...
This Information Applies To: Agilent G7167A/B Infinity II Multisamplers
Issue:
The Agilent Multisampler autoreferencing procedure uses predefined positions on the base plate and the sample hotel to calibrate the positioning of the needle parkstation...
This Information Applies To: Agilent 1260 Infinity Binary, Quaternary or Isocratic Pump Modules
Issue:
Reinstallation of the pump head on the pump metering drive during maintenance on an Agilent 1260 Infinity Binary, Quaternary or Isocratic Pump...