For Cell Mito stress test, Seahorse Cell Mito Stress Test Kit, the recommended working concentrations are 1.5 µM for Oligomycin and 0.5 µM for Rot/AA. The FCCP concentration needs to be optimized (0.5 µM-2 µM). The optimal final compound concentration…
In general, the %CV across a group and from plate to plate should both be < 20%. Higher CVs in this case was due to edge effect on outside of the plate.
GC/MS Photo Parts List for SQ and TQ sources
Stainless Steel, Inert, Extractor, HydroInert, Chemical Ionization, and the High Efficiency Source
These diagrams and parts lists are included in the Troubleshooting & Maintenance manual for the 5977 which…
The free Glycan Peak Assignment Tool was designed to provide additional confidence in identifying InstantPC or 2AB N-glycan peaks by predicted retention times of over 100 glycans. The new tool does not require users to upload sensitive data from N-glycan…
This Information Applies To: Agilent OpenLab CDS (version 2.x)
Contents:
In the following procedure, you will find steps to create a multipoint ESTD calibration method with OpenLab CDS 2.X.
Steps to follow:
1. Load the Data
a. From Data...
This Information Applies To: Agilent MassHunter Quantitative Analysis Software
Issue
Chromatographic peak integration is a critical part of accurate and reproducible quantitative data analysis. The integrator type and the integration parameters...
This Information Applies To: Agilent 6200/6400/6500 Series LC/MS Systems
Issue
Instructions on how to fill the calibrant bottle on 6200/6400/6500 Series LC/MS Instruments when calibrant levels are running low.
Background
The ability to autotune...
This Information Applies To: All Agilent 6000 Series LC/MS Systems without a gate valve for ion capillary installation/removal.
Issue:
Installing the Ion Transfer Capillary after cleaning or replacement. For instructions for installing an ion capillary...
This Information Applies To: AriaMx Real-time PCR System
Issue
The saturation level is unique for each optical module after the offset of calibration factor and background calibration. The FAM, ROX, HEX, CY3 & CY5 optical modules will saturate at...
This Information Applies To: Agilent LC Systems
Issue
While it is possible to combine modules from different HPLC series such as 1100, 1200, 1260 or 1290 series to create a functioning LC system, there are some important topics to consider.
...
Fixes for the "RPC Server Unavailable" Error
Multiple Results
This Information Applies To: Agilent OpenLab CDS
Issue
When launching the instrument Acquisition screen/instrument session, there is an "RPC Server Unavailable" error.
Resolution
Accessing the Services App (Windows)
There are two ways to...
This Information Applies To: Agilent LC Pumps
Issue
If a user suspects that the gradient composition isn’t accurate and causes poor chromatography such as retention time shifts, late, early, no peaks or merging peaks. This article provides a quick...
This Information Applies To: Agilent 1290 Infinity (G4226A) and Agilent 1260 Infinity (G1367E) HiP Autosampler
Issue
Replacing the Needle Seat of the Autosampler is required when:
The needle seat is visibly damaged.
Leaks or blockages in the...
This Information Applies To: Agilent OpenLab CDS
Issue
This article discusses different types of bracketing modes and how to set up a bracketed calibration in Data Analysis for OpenLab CDS 2.x
Background
Occasionally, the instrument conditions...