Analyze Agilent Seahorse XF Real-Time ATP rate assay data using Agilent Seahorse Analytics
Analyze Agilent Seahorse XF Hu T Cell Activation Assay data using Agilent Seahorse Analytics software
Analyze Agilent Seahorse XF Substrate Oxidation Stress…
Hello APacana ,
The reason for the difference in response between the TIC and the EIC is because when you extract the TIC of a given precursor you are extracting the ion signal from all precursors that create a 72 product ion, using your example. If…
Hello AJohnson13 ,
Here is the PC setup guide for older Windows 10 versions of MassHunter. It includes the PC specifications.
Windows10_Setup.book (agilent.com)
The software installation instructions would be included as part of your original media…
At 1 mL/min pumping water, the pressures are as follows:
Pump + Column = 53 bar
Pump + Column + 0.1 mm ID 30 cm tubing = 70 bar
Pump + Column + Tubing + Flow Cell = 75 bar
I have also confirmed that this pressure fluctuation has nothing to do…
It all depends on your following steps. You can even use Methanol or Acetonitrile for the protein precipition: Protein Precipitation for Biological Fluid Samples Using Captiva EMR-Lipid
See also the chapter on protein precipitation in Agilent's Handbook…
The Digest of upcoming webinars has been updated as of April 30.
Did you miss April's GC Live Session Recording and Discussion Thread? Check it out here: ( +) Diagnostics From Your Desk, Troubleshooting From Your Kitchen Table - Follow-up Discussion…
Hello cjwwfd ,
B.06.00 is out of support at this point, but I can offer some general suggestions for current revisions and hopefully you can apply them to your version. Version 12.0 is the current release. If possible, I would investigate your options…
I'm experimenting the same error. Logbook displays <MSQQQ_1: QqQ fault detected: 1.23 vacuum system is not ready. This is typical during a pump down cycle or when the high vacuum pump is turned off during a vent cycle or fault condition>. Does anyone…
Hello DET ,
Sorry for the late response on this.
What sort of spiking do you see usually? What is the output of the FID, in pA?
Are you using any UPS systems, or are the GCs plugged directly into the wall?
This Information Applies To: 5200/5300/5400 Fragment Analyzer, Fragment Analyzer controller software
Issue:
Over time the dynamic coating polymer on the capillaries needs to be refreshed. Failure to do so can lead to a loss of performance. Indicators...
This Information Applies To: Agilent ICP-MS (7700/7800/7850/7900/8800/8900)
Issue
Elevated doubly charged values that are greater than 3% for x-lens configuration or greater than 7% for s-lens configuration, can be caused by contamination, or an...
This Information Applies To: Agilent 6200 Series TOF and 6500 Series Q-TOF Systems
Issue
Instructions on how to correctly dilute the tune mix for 6200 Series TOF and 6500 Series Q-TOF systems depending on source type.
Background
If the Tune...
This Information Applies To: Agilent MassHunter Qualitative Analysis Software version 10.0
Issue
The Agilent MassHunter Qualitative software can generate qualitative analysis reports, which may include sample chromatogram information and sample...
This Information Applies To: Agilent MassHunter Qualitative Analysis Software version 10.0 or higher
Issue
Identifying compounds using a spectral library is essential in qualitative analysis. This article will describe how to load a data file and...
This Information Applies To: Agilent ICP-MS Systems
Issue
The Helium cell gas line on the ICP-MS may be contaminated with air and moisture in case of a tank replacement, or if the instrument was not used for an extended amount of time. Purging the...
This Information Applies To: Agilent MassHunter Quantitative Analysis Software
Issue
The Calibration Curve window tools help with setting an appropriate calibration curve for each compound in the calibration table.
The Calibration Curve window...
This Information Applies To: Agilent MassHunter Quantitative Analysis "Quant-My-Way" user interface (version B.09.00 or later)
Issue
To process the data files in the batch and obtain quantitative results, you must either create a new quantitative...
This Information Applies To: Agilent MassHunter Qualitative and Quantitative Analysis
Issue
Calculation of signal-to-noise in MassHunter is done using the same routine for both Qualitative and Quantitative Analysis. However, there are differences...
This Information Applies To: Agilent GC/MS MassHunter Data Acquisition Software
Issue
Multiple samples can be run without user intervention using an Automated Liquid Sampler (ALS) in combination with a Sequence.
Users can perform simple edits...