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  • Category :  Agilent Knowledge Portal
  • Entry Type :  Article
  • Product Type :  Real-Time PCR (qPCR)
  • Component :  qPCR Systems
  • Product Name :  AriaMx Real-time PCR System
  • Task :  Troubleshooting
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Mismatch Between the Selected Dyes and Optical Modules on the AriaMx

Answer

This Information Applies To: Agilent AriaMx real-time PCR system

Issue

When configuring a plate setup in the AriaMx software, it is crucial to ensure that the selected dyes correspond to the optical modules installed in the instrument.

Background

If there is a mismatch, the following error message will appear in the AriaMx software while priming of the experiment: The dyes configured in plate setup do not match with the optical modules detected at the instrument. Reconfigure the plate dyes at the instrument and try again (Figure 1).

 

Figure 1. Mismatch error message

Resolution

To identify the discrepancy, return to the plate setup screen in the AriaMx software. The filters not present in the AriaMx will be highlighted in red. The dyes that are selected for use in the assay will have a tick in the check box to the left of each dye name (Figure 2). If a dye has a tick in its check box and is highlighted in red, the dye is selected in the experiment, but the corresponding optical module is not installed.


Example of a missing filter display. The experiment is set up to use ROX and SYBR but ROX is one of the optical modules not installed in the instrument.
Figure 2. Example of a missing filter display. The experiment is set up to use ROX and SYBR but ROX is one of the optical modules not installed in the instrument.

To resolve this issue, you have two options:

  • Install the required optical module: how to install an optical module is described in the article Inserting and Removing AriaMx Optical Modules.
  • Modify the experiment: modify the plate setup by selecting dyes that match the optical modules currently installed in your AriaMx.

After you have installed the required optical modules or changed which dyes are selected, prime the experiment.

  

To ensure optimal performance of your AriaMx, choosing the right optical modules is essential. For a comprehensive guide on selecting compatible plasticware and modules, please see the article: Which Fluorescent Dyes can be Detected with the AriaMx?

 

  
Caution: Improper handling of optical modules can result in damage, such as scratches that impair performance. For detailed guidance on preventing and addressing such issues, see the article Scratches on AriaMx Optical Modules.

 

 
Learn how to effectively operate your Agilent AriaMx:
AriaMx Real-Time PCR System Setup and User Guide on Agilent.com
Aria Real-Time PCR Software User Manual on Agilent.com

For Research Use Only. Not for use in diagnostic procedures.

PR7001-2548

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