• Created : 
  • Modified : 
  • Category :  Agilent Knowledge Portal
  • Entry Type :  Article
  • Product Type :  LC/MS Systems Mass Spectrometry Software
  • Component :  LC/MS Data Acquisition Software LC/MS Single Quadrupole OpenLab Chromatography Data System
  • Product Name :  6130 Single Quadrupole LC/MS 6140 Single Quadrupole LC/MS 6150 Single Quadrupole LC/MS InfinityLab LC/MSD InfinityLab LC/MSD XT InfinityLab LC/MSD iQ OpenLab CDS
  • Task :  Operation
Related Articles

  • Venting and Shutting Down your Single or Triple Quadrupole LC/MS System

    Multiple Results
    This Information Applies To: Agilent 6100 and 6400 Series LC/MS Systems controlled by OpenLab CDS ChemStation or MassHunter Workstation for LC/QQQ Issue How to safely vent and turn off your Agilent Single or Triple Quadrupole LC/MS system. ...

  • Checking the Instrument Status of your Agilent Single Quadrupole LC/MS System Using OpenLab CDS

    This Information Applies To: Agilent Single Quadrupole LC/MS systems controlled by OpenLab CDS software Issue How to check and recognize the current Instrument Status of your 6100 Series and InfinityLab LC/MS systems using OpenLab CDS software. Steps to Follow If necessary, in the top ribbon, click the Home tab, the click Instrument Status. Figure 1 . Instrument Status option location In the Instrument Status window, click the Dashboard tab to see the SQ Instrument Status...

  • Why is my Single Quadrupole LC/MS Not Ready?

    This Information Applies To: Agilent 6100 series Single Quadrupole (SQ) LC/MS Instruments using OpenLab CDS software Issue Waiting to run a sample or tune the instrument but the MS is shown as not ready. Background The MS requires accurate temperature, gas flow, and electronic control in order to maximize performance. If one of the MS setpoints is not yet reached, or has not stabilized, then the MS is shown as "Not Ready" and cannot analyze samples or tune. This status is to prevent the...

  • Filling the Calibrant Bottle in Agilent 6100 Series Single Quadrupole LC/MSD Systems

    This Information Applies To: Agilent 6100 Series LC/MS Instruments Issue Instructions on how to fill the calibrant bottle in Agilent 6100 Series LC/MS Instruments when calibrant levels are running low. Background The ability to autotune your Agilent LC/MS system relies on providing the instrument with the correct composition and concentration of calibration solution. This article provides detailed instructions on how to make up your required calibration solution and refill your calibrant...

  • Checking the Calibrant Level in your Agilent LC/MS System

    This Information Applies To: Agilent LC/MS System Issue Instructions on how to check the level of calibrant in your LC/MS system, and refill the calibrant bottle if necessary. Requirements Checking the level of calibrant should be performed weekly, or more often if frequent instrument tuning is required. Steps to Follow Open the door or remove the cover at the front of the instrument to reveal the calibrant bottle Carefully check that the intake tube that draws up calibration...

Turn off your Single Quadrupole LC/MS System Using OpenLab CDS

Answer

This Information Applies To: Agilent 6100 Series single quadrupole LC/MS systems controlled by OpenLab CDS Software

Issue

Instructions on how to safely turn off the Agilent 6100 Series or InfinityLab Series LC/MS System

Steps to Follow

  1. Turn off the LC solvent flow.

      
    Caution: If you have analyte in any of the components of the LC flow path, flush the flow path with pure solvent before you continue, then turn off LC flow once flushing is complete.

  2.  Put the MS into standby state. (See Put your Single Quadrupole LC/MS Instrument into Standby Using OpenLab CDS)

  3. Wait for the MS to reach standby setpoint temperature and gas flows.

  4. In the Instrument Status window, click the Dashboard tab.

  5.  Right click the SQ Instrument Status Pane, and click Vent (See Figure 1)


    Figure 1
    Figure 1. Vent Command in SQ Status Pane
      
    Caution: You are shown a Caution message before you can Vent the system. It can take several hours to re-establish the proper vacuum levels and to re-equilibrate temperatures if there is an unwanted vent. Check if the MS will not be needed for any further analysis before venting.

  6. To vent the instrument, click Yes to proceed with venting.
    During the vent cycle, the following occurs:

    • Spray chamber voltages, the drying gas heater, the nebulization gas flow, the detector, and other specific lens voltages are all turned off.

    • The drying gas flow is set to standby.

    • If the APCI source is installed, the vaporizer heater is also turned off.

    • If an Agilent Jet Stream source is installed, then the sheath gas heater is also turned off.

    • The Rough Pump turns off when the turbo pump speed is below 20%

  7. Wait until the instrument completely vents, reaching a rough vacuum reading of 760 Torr, or atmospheric pressure. The vent process normally takes less than 10 mins and the rough vacuum readings can be monitored via the SQ tab instrument actuals. Scroll down to see the rough vacuum reading.

  8. Once the instrument is vented, close the OpenLab Online session, turn off the instrument using the front power switch.

       Warning: Certain internal components are energized whenever the main power cable is connected even if the front power switch is turned off, so it is always safer to unplug the main power cable after venting.

  9. Unplug the main power cable connecting the MS to the power outlet to de-energize the MS

  10. If necessary, the LC modules can now also be switched off at the power switch at the front left bottom corner of each LC module.

 

 
Learn how to effectively operate your Agilent Single Quadrupole LC/MS:
Agilent Single Quadrupole LC/MSD with OpenLab CDS e-learning path available from Agilent education
Was this helpful?
Privacy Statement
Terms of Use
Contact Us
Site Help