Fragment Analyzer 5200: Missed Dilution 5 times 930dsDNA Inlet buffer

Good morning Nadya and thank you for using Agilent Community.

regarding of the issue you are facing I would recommend you to perform a Method C flush and/or a tip soak ( see fragment analyzer system manual for step by step procedure) to restore the capillary array. This mistake shouldn't have any impact on your capillaries nevertheless in case of a run with the concentrated buffer you should expect to see much higher separation current and some separation issues.  

regards

Matthieu Claustre 

EMEAI Remote Support Engineer - Genomics

Hi Nadya, Follow up to above comment, as stated, details on Capillary array cleaning by Method C and Method B (tip soak) can be found in Fragment Analyzer manual starting page 148. Thank you, Shweta 

Hi Nadya, Follow up to above comment, as stated, details on Capillary array cleaning by Method C and Method B (tip soak) can be found in Fragment Analyzer manual starting page 148. Thank you, Shweta