Fragment Analyzer 5200: Missed Dilution 5 times 930dsDNA Inlet buffer

Good morning Nadya and thank you for using Agilent Community.

regarding of the issue you are facing I would recommend you to perform a Method C flush and/or a tip soak ( see fragment analyzer system manual for step by step procedure) to restore the capillary array. This mistake shouldn't have any impact on your capillaries nevertheless in case of a run with the concentrated buffer you should expect to see much higher separation current and some separation issues.  

regards

Matthieu Claustre 

EMEAI Remote Support Engineer - Genomics

Hi Nadya, Follow up to above comment, as stated, details on Capillary array cleaning by Method C and Method B (tip soak) can be found in Fragment Analyzer manual starting page 148. Thank you, Shweta