EIC are flat-lined where peaks are expected. Probably something simple but I'm at a loss how to proceed. TIA!
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EIC are flat-lined where peaks are expected. Probably something simple but I'm at a loss how to proceed. TIA!
Hello,
First, you should extract accurate mass, with 3 or 4 decimal places. Nominal mass will not work for HRMS. You can change extraction window width at the "extract chromatogram", where you set an extracted mass. It is usually in ppm, I would recomend 20 or 40 ppm.
Even better is to use "Find-by-formula", where you paste formulas of your analytes and it will automatically calculate exact mass of different adducts, extract them and qualify also isotopic pattern.
There still might be a problem with a mass accuracy (shifted masses) or something else. But exact masses are essential.
Ondrej
Hello,
First, you should extract accurate mass, with 3 or 4 decimal places. Nominal mass will not work for HRMS. You can change extraction window width at the "extract chromatogram", where you set an extracted mass. It is usually in ppm, I would recomend 20 or 40 ppm.
Even better is to use "Find-by-formula", where you paste formulas of your analytes and it will automatically calculate exact mass of different adducts, extract them and qualify also isotopic pattern.
There still might be a problem with a mass accuracy (shifted masses) or something else. But exact masses are essential.
Ondrej