Masshunter Quantitative analysis version B.09.00 not generating peak area under EIC.

Question

Hi all, 
 I am using Masshunter quantitative version B 09.00 (for TOF) to analyze some scan GC MS data from my GC QTOF. I would like to find out the peak areas for my ions of interest under extracted ion chromatograms, but the EIC results from masshunter quant show 'no data points' (as shown in the image below). 
 I converted my GC QTOF data into suremass and TDA format, but it shouldn't influence the analysis in Masshunter Quant. 
 
 Any suggestion to make it work? Thanks!

howard_sanford · Answer

Hello May01 , 
 There are a few things you can check. First check your MZ and your mass extraction window to make certain there is signal present. This is an extreme example, but you can see that there will be no data points extracted if the MZ or the extraction range is not set appropriately.

I would also check your signal without TDA conversion and with SureMass disabled, as in certain cases there can be issues with feature extraction and indexing that can cause signal issues. If TDA information is present quant will use this by default. SureMass will only be used if it is enabled in the Method Global Setup section. To check your data without either feature, first make certain that SureMass is disabled in your method.

Then you can go to the Tools->Convert Accurate Mass samples menu and choose to Convert to SureMass format and Delete TDA files. You may also need to choose Always convert even if data is already converted. 
 
 You can also manually delete the TDA files from the data folder, but I would suggest making a backup of your data files before trying this to be safe. The files to delete are located in the AcqData folder within the .d folder. 
 TDA.bin 
 TDASpec.bin 
 TOFDataIndex.bin 
 
 When reloading the batch make certain to select No when prompted to perform TDA conversion.