Displaying LOD LOQ mass Hunter Quantitative 10.2

Hello Lstephan ,

To populate the method LOD and LOQ columns you need to assign a limit to the method outlier. This can be done in the Method Editor under Outlier Setup.

After a method limit is set it will be displayed in the batch table. The flag will be displayed on the Calc. Conc. entry.

Thanks a lot, Howard 
for your response.

I was thinking thaht Mass Hunter was able to calculate LOD and LOQ from linear regression.
In fact, we have to calculate them and fixe our limits to display flag.

I would be interesting that Mass Hunter calculate them and that we could choose from different methods to calculate.

Lstephan ,

I'm not aware of a way to have quant calculate the LOD or LOQ from a linear regression or calibration curve.

There is a script/wizard that will calculate the MDL, LOQ, and/or LOD from replicate injections. It can be found in the Tool menu under Actions. If you have Quant Supplemental installed it includes a video that goes into detail about how the script can be used and what method it uses to calculate the MDL/LOQ/LOD.

D:\MassHunter\Videos\Quant\Quant scripts - MDL-LOQ-LOD - Wizard.mp4

The script itself is located here by default - 

D:\MassHunter\Scripts\Quant\Actions\MDL-LOQ-LOD.quant.script

You would want to make certain that this uses the same method and criteria for calculating the MDL/LOQ/LOD that your lab or method procedures have established. Some parameters that the script uses can be adjusted. From the comments of the script - 

//This script calculates the following from replicate injections:
//• Method Detection Limit (MDL)
//• Limit of Quantitation (LOQ)
//• Limit of Detection (LOD)
// SampleType can be "Sample", "QC" or "Calibration"
//Step 1: Configure the MDL-LOQ-LOD script: Open MDL-LOQ-LOD.quant.script in Notepad,
// in the “User configuration” section, enter the desired values for ConfidenceLevel
// and BlankSubtration. Please note:
// 1) Any Confidence Level and Degree of Freedom is supported.
// 2) If the Blank subtraction is turned on, the average concentration
// of BLANK (include Blank and DoubleBlank) samples will be subtracted
// from average concentration. All other concentration based calculations
// such as MDL, LOQ, LOD, Concentration RSD, etc are based on the adjusted
// concentrations.

//Step 2: Load and analyze batch

//Step 3: Run the script: Click Tools\Actions\Custom Action…, select the MDL-LOQ-LOD.quant.script to run.

//If the script is run in the Method Editing mode, “Set MDL”, “Set LOQ” and “Set LOD” buttons are available
//and clicking on it will set corresponding outlier for the selected compounds.

Thanks a lot for all those informations.
I ll try it as soon as possible.

Best regards.

Ludovic

Thanks a lot for all those informations.
I ll try it as soon as possible.

Best regards.

Ludovic