Hi, LCMS users,
I am using Agilent 1260 II, got a strange problem about the pressure, the details are described below:
Before start to run a worklist, we will stabliazed the system with the mobile phases, their proporation, flow rate, and both ends temperature of the seperation column are the same as the initial condition as defined in the employed acqusition method. After the LCMS system stablized, we will recod the quaternary pump pressure and start to run the worklist.
So, the problem is the stablized pressure is untable between runing different worklist. For example, the stablized pressure is 270bar, whilst next time it is 330bar, and colud jump to 280bar next time.
Anyone can share some insights into this problem? thanks a lot.