Hello everyone
I am trying to couple Agilent 7100 CE with Agilent 6230 ESI-TOF-MS with the following parameters:
CE parameters:
Capillary: PVA, 65 cm, 50µm i.d. (CE/MS)
Sample: IgG1 (3mg/ml)
Injection: 50 mbar, 5 seconds
Buffer: 2% acetic acid
Voltage: 30 kV
Temperature: 20 degree Celsius
MS parameters:
Ionization mode: Dual ESI
Acquisition mass range: 1000 -6000 m/z
Sheath liquid: 0.5 % acetic acid in 50 % methanol, 5 μL/min
Drying gas flow: 5 L/min
Nebulizer: 10 psi
Drying gas temperature: 250 °C
Fragmentor: 350 V
Vcap: 3,500 V
The CE current is stable and there isn’t any problem with MS tuning and calibration. Everything seems to work fine yet I am constantly getting the message “Spectra cannot be displayed” and no data is recorded. Tried restarting the software and the PC, increasing nebulizer, changing sheath liquid flow rate but nothing worked. Am I missing something? Please help.
