Changing nitrogen gas supply for collision cell

Hello,

I'm about to replace the nitrogen tank that feeds the collision cell on our 6545 QTOF. I just have a simple question, can I change the nitrogen tank while the MS is in stand-by mode or does it need to be vented ?

This brings me to a second question : What is the use of nitrogen for CC if no MS/MS analysis is performed ?

Have a nice day

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