Auto tune fails: Cannot perform coarse Mass Calibration?

We work with a Q-TOF 6545 and it fails positive auto tune. Is there anyone who knows this error, what causes it and what can be done to rectify this?

Thank you

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  • Hi ,

    I always recommend to customer to look at the  tuning solution in positive first (if you have a TQ, go in MS2 scan) as you will see if you have any contamination. A good tell-tale of a degraded tuning solution is the presence of unknow peaks, low abondance's on tuning peaks, etc.

    When looking at the tuning peaks, you also want to make sure that you do not have other masses interfering with your compounds of interests. Mass 118 and 322 are more susceptible to have contamination close by.

    I hope this helps.

    Regards,

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