asking for your opinion on optimizing

  1. Dear. LC/MS scientists

    I truly need your help.
    I have optimized ginsenoside Compound K (C36H62O8, 622.44) for 2 weeks. I was using MH Optimizer program. I confirmed that the precursor ion and product ion was same as article (621.4/161). The injection condition was 10 ug/ml, 2 ul.
    I use the negative method. precursor Ion abundance was 60000, product Ion was 1500. I know that negative conditions are generally lower than positive conditions. But I can’t figure out why I can’t check the linearity.
    How can I solve this problem?

    I'm sure it's not a hardware issue because the machine was inspected two weeks ago.

    Thank you

Was this helpful?