Hello all
I am experiencing an unstable baseline problem increasing with increasing MeOH in the gradient and sudden drops in baseline.
This is a typical plasma metabolome profiling experiment with 1290 UHPLC and 6546 Q-TOF in NEGATIVE ION MODE
A: H20 (0.1 %Acetic acid) B: MeOH ( %0.1 Acetic acid)
Column ins HSS T3 Xbridge
As you see this is more like a IR spectra. Nebulizer and system temps are all OK.
Would incrasing AcA to 1% help?
Spectrum:
The gradient program is:
and method parameters are:
Any help and suggestions are much aprreciated.
Basri
