Masshunter Quantitation using Surrogates

I need to quantify surrogate (extracted ion standard) by Internal standard and target compound by surrogate, How to make the same in Mass Hunter software.

Could any one answer the same?


  • Hi Binu,

    I write step by step what you need to choose in the software. To open  massshunter qua,n soft- open batch- clıck method- choose ınternal standar setup top on the left side- you see compound name- the part of the target in the related articles is changed to surragate and istd- "none", you will be able to choose surrogates and ISTD-  click ISTD flag-write ISTD concentratıon

    That's all. 

    I hope the instructions were descriptive.

    Best Regards

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