Our lab currently has a 6546 QTOF. I recently watched an Agilent webinar about tuning QTOF's and they mentioned that the skimmer voltage for the 6545, 6546, and 6545XT should be 45V versus 65V for other systems while tuning. Does anyone know why this is the correct voltage? All our methods currently use 65V as the setting. Will tuning the system at 45V but running all our acquisition methods at 65V have a negative impact on the data? And should we be running at 45V instead?