I typically try to clean up and buffer exchange samples into ammonium acetate or (worst case) PBS; however, I need to compare some results with either 50mM HEPES or 20mM Tris.
Wondering if anyone has suggestions as to which one is worse in terms of contamination and/or buildup?
To give some extra detail:
- This is on a 1290/6545XT LC/MS w/ AJS
- Running intact protein MS with Water:ACN+FA
- I use a PLRPS column and inject ~1 uL.
- I divert the first 1 min to waste, in order to help "wash" the sample