LC-MS tune report justification for low mass quantification


We are working on the quantification of nitrosamines with LC-MS/MS (Model: 6470). The mass calibration was performed by autotune with the following low mass.

  • Q1 for Positive: Lower mass 118.09
  • Q1 for Negative: Lower mass 112.99
  • Q2 for Positive: 58.07
  • Q2 for Negative: 69.00

How do we justify the quantification of low molecular mass compounds (Below 100 for Q1 & Below 50 for Q2) with the same tune report..?

With regards,

Bhargava J.

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