QTOF 6545 not seeing reference mass

Hi Simon,

When you turn on the reference mass in the software, do you see it spraying from the ref sprayer in the source?

We did find a blockage in the line between the bottle and nebuliser, which we flushed with a syringe. Doing the flush has allowed us to see the reference mass again, though the abundance is 3 orders of magnitude less than expected. We are using a fresh solution from a stock preparation made in September.

The instrument was off for a period due to an unrelated failure in the lab's UPS. 

I can not see any spray currently and we are thinking that the nebuliser, may also have a partial blockage.

Tubing should be replaced, part number G1969-60115 on Agilent.com.