Injector program for Dwell volume correction

Hello,

I want to make transfer from one system to another that has higher dwell volume.

What I know is that the new system should have injection time delay to prevent dwell volume incompatibilities.

I have two questions:

1) In some HPLC C.01.08, C.01.09 are installed, I see that injection program button is available on it, but when I have started an analysis, injection program did not work (I have tried both, single run and sequence, but nothing worked)

2) Could you give me some advise how to modify injection program to inject with delay, while pump starts on its time.

3) How can I calculate dwell volume in an way Agilent calculates.

Thank you.

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  • Hello,

    I Have performed Dwell Volume calculation with the attached method.

    I calculated dwell volume in some different ways.

    1) Saved CSV file from Lab Advisor and calculate 50% of height from it. I got 1186mkL.

    2) From This tools attached above, found 50% of height and zoomed in. I got 1149mkL.

    Would you recommend some techniques to determine Dwell volume in an accurate and conventional way?

Reply
  • Hello,

    I Have performed Dwell Volume calculation with the attached method.

    I calculated dwell volume in some different ways.

    1) Saved CSV file from Lab Advisor and calculate 50% of height from it. I got 1186mkL.

    2) From This tools attached above, found 50% of height and zoomed in. I got 1149mkL.

    Would you recommend some techniques to determine Dwell volume in an accurate and conventional way?

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