Baseline drift using THF

I am getting a massive baseline drift of 1200 mAu when I use THF as my organic solvent. I do not get that with acetonitrile. I run a gradient from 50/50 to 100% THF over 7 min. The baseline drift starts after ~2 min when the THF content is is at ~60%. I get it with reference (bandwidth 100 nm) on or off. How do I correct so I get a flat baseline?

Parents

0 kbusch 1 month ago

What is the wavelength? THF can absorb at lower wavelengths such as 210 nm. Maybe you are just seeing the mobile phase absorb.
Cancel
Vote Up 0 Vote Down

Sign in to reply

Verify Answer

Cancel
0 KHerault 1 month ago in reply to kbusch

Thanks for the reply. I am monitoring at 254 nm. Higher wavelengths do not show that drift. It must be related to the THF (I am using unstabilized) but I do not see how.
Cancel
Vote Up 0 Vote Down

Sign in to reply

Verify Answer

Cancel

Reply

0 KHerault 1 month ago in reply to kbusch

Thanks for the reply. I am monitoring at 254 nm. Higher wavelengths do not show that drift. It must be related to the THF (I am using unstabilized) but I do not see how.
Cancel
Vote Up 0 Vote Down

Sign in to reply

Verify Answer

Cancel

Children

No Data

Was this helpful?