Baseline noise and drift issue. G4212B Max light.

Dear team,

We have an G4212B DAD detector. We have an issue with baseline. Please see below. It's water 1 ml/min. 254 nm (4mm), reference 500 (100mm)

below results flow off, Max light Cell 10 mm.

below results flow off, Max light Cell 60 mm.

We flushed the flowpath for two days: IPA, MeOH and water. We have tried with column and with zero dead volume fitting. 

What could be the possible source of such  drift and noise in baseline?

Thank you all in advance.

Kind regards,
Rustam

Parents Reply Children
  • The best way to troubleshooting signal issues is to separate the flow path from the light path as good as possible.

    With your detector this would require the use of the dedicated Max-Light cartridge Test Flow Cell # G4212-60011.

    In this way you can monitor the detector signal and verify if the impact coming from the flow cell somehow or from the light path and light source.

Was this helpful?