.Hi all,
I am new to Agilent LC systems and I want to develop an alternating-column regeneration method.
I have an Agilent 1290 II system with 10 port valve column compartments. I know this method requires two pumps so can I use the Cap pump as a second pump although the maximum flow rate is 20 uL? or there is any way to use the 1290 Infinity II High-Speed Pump only?
Also, I need help on how to build the method on the chemstation software.
Thanks,