Aminoacidos por HPLC - FLD

Question

Estoy realizando an&aacute;lisis de amino&aacute;cidos, con frecuencia en el minuto 12 sale un poco con una altura considerable. &iquest;Cu&aacute;l podr&iacute;a ser la causa?

kbusch · Accepted Answer

Maybe you can try a strong column cleaning step towards the end of the method to remove anything from the stationary phase. That means likely from HPLC-FLD amino acid analysis going to a 100 percent organic concentration after the gradient is finished and then re-equilibrating for starting conditions prior to the next analysis.

Alindor · Answer

Ok 
 Muchas gracias 
 El final de la gradiente es 100 % de una mezcla de acetonitrilo:metanol:agua (45:45:10) por 5 minutos a 1.5 ml/minuto 
 Consulta, c&oacute;mo debo re equilibrar?

Aminoacidos por HPLC - FLD

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