Maximum Buffer concentration in HPLC and UHPLC Agilent

Usually standard buffers work well on our instruments. However, I would strongly recommended that you always flush the entire LC thoroughly with fresh water and store it in IPA after you're done with the analysis. Keep the physical boundaries in mind to prevent precipitation (e.g. a KH2PO4 buffer of 20 mmol (or higher) concentration will form crystals at an organic level of 80-90%). 

Usually standard buffers work well on our instruments. However, I would strongly recommended that you always flush the entire LC thoroughly with fresh water and store it in IPA after you're done with the analysis. Keep the physical boundaries in mind to prevent precipitation (e.g. a KH2PO4 buffer of 20 mmol (or higher) concentration will form crystals at an organic level of 80-90%).