Hello,
The absorption at 230nm seen in the Online Signals view when I am operating the HPLC increases steadily over the course of an injection runtime. The set up of the analysis is as follows in this literature. https://www.agilent.com/cs/library/applications/application-dedicated-cannabinoid-potency-testing-5991-9285-en-us-agilent.pdf
Both Methanol Blanks and Standards in methanol end up increasing in absorption at around the midpoint of the runtime, then plateaus and goes to 0 at the end of the runtime. This increase in absorbance coincides with a increase in pressure over the course of a run.
We run it 20-30 times and each 9.50 min runtime looks the same.
