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Bad pump that passes diagnostic tests?

aew

Hey guys,

Our gradient test has been failing, got a new MCGV, replaced inlet and outlet filters, rotor seals, frits, switched degassers, switched lamp, cleaned flow cell, and still get a lot of drift...particularly when more than one channel is used. Pump pressure and leak tests Pass in LabAdvisor.  See attached pic.  All channels are in the same mobile phase, with varying ratios (25 to 75%) in 30 increments, A with B, C with D. Anyone ever seen this, and how to remedy?  

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    Hello,

    I am not sure what gradient test you are running.  You said that all channels have the same mobile phase in them.  Normally, a gradient test is run with two channels one of which has solvent and the second has the same solvent with a small amount of tracer.  For example:  Channel A is water and Channel B is water with 0.5% Acetone.

     

    If you are using a tracer and cannot resolve your gradient issue, you may want to have onsite service.

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  • 0

    Hello,

    I am not sure what gradient test you are running.  You said that all channels have the same mobile phase in them.  Normally, a gradient test is run with two channels one of which has solvent and the second has the same solvent with a small amount of tracer.  For example:  Channel A is water and Channel B is water with 0.5% Acetone.

     

    If you are using a tracer and cannot resolve your gradient issue, you may want to have onsite service.

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