I am new to HPLC and am using our Agilent HPLC 1200 series (combined with newer components) with ChemStation software version C.01.07.
We are using our HPLC for protein purification and possess both a DAD and an FLD. The proteins are sometimes, but not always, fluorescently labelled, meaning that we usually do not need the FLD. We want to minimize the use of the lamp to prevent its ware, but now whenever I want to run a sample the run does not start unless the FLD is on. Does anyone know how to prevent this, so that the FLD is only on if the method states that an FLD signal must be collected?
Thank you very much for your advice and help!
All the best,