hii, I am working with RP C18 column in Agilent 1260 infinity II, I am using KH2PO4 as buffer which runs in gradient with Acetonitrile. 5 days back my blank (HPLC MQ) was clean. However now there are ghost peaks of height 30-200 mAU at 211 nm for blank MQ sample every time I am running it and at same retention time. can you please tell me what is wrong and how to resolve this.
