Atypical Chromatogram

Hello, we have observed a couple of atypical chromatograms in the same run.  In a run sample injections, the 11th and 13th injection were observed to be atypical.   We have looked into the parameters of each injection and they are within our method specifications, also the pressure has been constant throughout the sample injection.  Any advise or next steps to figure out what is happening would be really helpful.

Thank you all! :-)

Parents
  • I agree with  that this issue seems to be restricted to the first couple of peaks. The rest of your chromatogram seems to match well. Overlaying the chromatograms would help with visualization. The MCGV could be the issue, as this would change the %organic in your system if it's not functioning properly. Another possible cause could be you sample. Is it being temperature controlled? Is it possible that the sample is degrading by the later injections?

Reply
  • I agree with  that this issue seems to be restricted to the first couple of peaks. The rest of your chromatogram seems to match well. Overlaying the chromatograms would help with visualization. The MCGV could be the issue, as this would change the %organic in your system if it's not functioning properly. Another possible cause could be you sample. Is it being temperature controlled? Is it possible that the sample is degrading by the later injections?

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