Baseline Drift Observed in AG1260 DAD Detector

Hello,

During the Gradient run in HPLC AG1260. one of the sample injection baseline is drifted at 18th minutes (total run is 70 minutes) . because of the baseline drift one unknown impurity at RT “43.912 minutes” and RRT “1.718”  is response higher and it is not in the trend. reinjected the same vial and found that the sample Unknown is in trend with the previous sample analyzed.

Note: Preventive Maintenance (Lamp Changed) was completed just before the problem.

Gradient Program for reference

Time

(minutes)

Mobile Phase-A (%)

Mobile Phase-B (%)

0

74

26

18

74

26

32

55

45

48

30

70

60

30

70

62

74

26

70

74

26

  • Hi

    Please share the model number of your DAD and the flow cell part #.
    What wavelengths are used in the above chromatograms?
    What were your mobile phases and is the temperature controlled in the column compartment?
    Does the baseline drift at 18th minute consistently appear in subsequent injections of sample even after conditioning the system for longer?

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